Dogs can detect cystic fibrosis bacterial pathogens at ultra-low concentrations, study finds
15 August, 2019
Trained medical detection dogs can differentiate between Pseudomonas aeruginosa (Pa) and other bacterial pathogens that commonly affect people with cystic fibrosis (CF) by sniffing bacteria grown in a nutrient liquid, according to a new study published in the European Respiratory Journal.
The researchers aimed to assess whether trained dogs could accurately distinguish Pa from other bacteria that are common in CF by scent, as it is hoped that this method could offer a more affordable and less invasive method of screening lower airway infections in CF patients.
To test this, the dogs were trained and tested on customised sample presentation stands. During training, correct identification of Pa samples was rewarded. Once trained, dogs were presented with Pa-positive cultured samples, other cultured bacterial controls or sterile liquid in a random, computer-generated sequence. A positive indication (when the dog either stopped or sat down) was rewarded if correct.
The samples were then presented in a double-blind testing scenario. Four blind studies were undertaken, including:
- Pa versus other bacteria familiar to the dogs, producing a mean sensitivity (correct signalling at a Pa sample) of 94.2% with a specificity of 98.5%;
- Pa versus previously unencountered bacteria, with two of the three dogs maintaining sensitivity at above 90%;
- Dilution testing; at dilutions of 1:1,000, sensitivity (93.8%) and specificity (94.9%) were consistently maintained;
- And mixed, multi-organism cultures, still correctly identifying Pa with a sensitivity of 86.5% and a specificity of 84.1%.
The researchers say these results demonstrate how the trained dogs can accurately detect possible indicators of infection and disease with a high level of accuracy.
Professor Jane Davies from Imperial College London was a lead researcher on the study. She said: “This is a really exciting development. Advanced technology to detect infections, for example in breath, has proved difficult so far. The successful training of the dogs on cultured samples will now be used as the foundation for testing patient samples directly.
“People with CF could ultimately monitor their lung infections from home by sending in samples for the dogs to check. We are very grateful to those with CF, cared for by Royal Brompton Hospital, for providing samples for this project and to the CF Trust for their funding support of the next stage of this project as part of the Strategic Research Centre for Pseudomonas infection.”
With the support of the CF Trust, the research team plan to test the feasibility of using medical detection dogs to identify infection from patient samples using clinical samples such as sputum, cough tissues and breath.
- Access the paper in the European Respiratory Journal
- Find out more about the Strategic Research Centre for Pseudomonas Infection in Cystic Fibrosis at Imperial College London
Pictured: Trained detection dogs Lizzie, Flint and Oakley (left to right) who took part in the study. Photo provided by Medical Detection Dogs.